Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1 around the phosphorylation site of Thr236. AA range:206-255
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
42kD
GeneID（Human）:
1901
Human Swiss-Prot No:
P21453
Cellular localization:
Cell membrane ; Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization.
Background:
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016],
Western blot analysis of HEPG2-UV MOUSE-BRAIN using p-EDG-1 (T236) antibody. Antibody was diluted at 1:500
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using S1P Receptor EDG1 (Phospho-Thr236) Antibody
Immunofluorescence analysis of COS7 cells, using S1P Receptor EDG1 (Phospho-Thr236) Antibody. The picture on the right is blocked with the phospho peptide.