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ELK Biotechnology
SKU(재고 관리 코드):ELK046ES
EasyStep Mouse PG(Progesterone) ELISA Kit
EasyStep Mouse PG(Progesterone) ELISA Kit
Alternative Names: P4; Pregn-4-Ene-3,20-Dione
Assay Type: Competitive Inhibition
Sensitivity: 0.7 ng/mL
standard: 100 ng/mL
Detection range: 1.56-100 ng/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PG antigen, and the Mouse PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse PG in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 0.7 ng/mL
standard: 100 ng/mL
Detection range: 1.56-100 ng/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PG antigen, and the Mouse PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse PG in the samples is then determined by comparing the OD of the samples to the standard curve.
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