1
/
의
1
ELK Biotechnology
SKU(재고 관리 코드):ELK0692
Cattle LPL(Lipoprotein Lipase) ELISA Kit
Cattle LPL(Lipoprotein Lipase) ELISA Kit
Alternative Names: LPL
Assay Type: Sandwich
Sensitivity: 0.27 ng/mL
standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal Transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LPL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LPL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LPL in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P11151
Assay Type: Sandwich
Sensitivity: 0.27 ng/mL
standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal Transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LPL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LPL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LPL in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P11151
Share
