1
/
의
1
ELK Biotechnology
SKU(재고 관리 코드):ELK0843
Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
가격·납기 및 기술자료를 확인해 드립니다.
제품 규격과 수량을 선택한 후 견적을 요청해 주세요. 대체품 검토와 SDS·COA·데이터시트 요청도 함께 접수할 수 있습니다.
이 제품 견적 요청대표번호 070-8028-3500 · info@rndmate.com
Alternative Names: 25 Hydroxy Vitamin D;
Assay Type: Competitive Inhibition
Sensitivity: 0.93 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 0.93 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Share
