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ELK Biotechnology
SKU(재고 관리 코드):ELK0857
Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit
Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit
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Alternative Names: RPS12; MRPS12; MRPS34; PSMD7; HTR1B
Assay Type: Competitive Inhibition
Sensitivity: 0.28 ng/mL
standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 2.5h
Research Area: Developmental science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O15235
Assay Type: Competitive Inhibition
Sensitivity: 0.28 ng/mL
standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 2.5h
Research Area: Developmental science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O15235
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