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ELK Biotechnology
SKU(재고 관리 코드):ELK0894
Pig ANG1(Angiopoietin1) ELISA Kit
Pig ANG1(Angiopoietin1) ELISA Kit
Alternative Names: ANG1; AGP1; AGPT; ANG-I; ANGPT1; Angiopoietin 1
Assay Type: Competitive Inhibition
Sensitivity: 1.15 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Tumor immunity;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig ANG1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ANG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ANG1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 1.15 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Tumor immunity;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig ANG1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ANG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ANG1 in the samples is then determined by comparing the OD of the samples to the standard curve.
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