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ELK Biotechnology
SKU(재고 관리 코드):ELK0974
Mouse GLO1(Glyoxalase I) ELISA Kit
Mouse GLO1(Glyoxalase I) ELISA Kit
가격·납기 및 기술자료를 확인해 드립니다.
제품 규격과 수량을 선택한 후 견적을 요청해 주세요. 대체품 검토와 SDS·COA·데이터시트 요청도 함께 접수할 수 있습니다.
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Alternative Names: GLY-I; GLOD1; Glyoxalase Domain Containing 1; Lactoylglutathione lyase; Aldoketomutase; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase
Assay Type: Sandwich
Sensitivity: 30 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GLO1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GLO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GLO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GLO1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9CPU0
Assay Type: Sandwich
Sensitivity: 30 pg/mL
standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GLO1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GLO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GLO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GLO1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9CPU0
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