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ELK Biotechnology
SKU(재고 관리 코드):ELK10333
Human KNG1(Kininogen 1) ELISA Kit
Human KNG1(Kininogen 1) ELISA Kit
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Alternative Names: BDK; KNG; HMWK; Alpha-2-Thiol Proteinase Inhibitor; Bradykinin; Kallidin; Fitzgerald factor; High molecular weight kininogen; Williams-Fitzgerald-Flaujeac factor; Ile-Ser-Bradykinin
Assay Type: Sandwich
Sensitivity: 2.31 ng/mL
standard: 500 ng/mL
Detection range: 7.81-500 ng/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KNG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KNG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KNG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KNG1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01042
Assay Type: Sandwich
Sensitivity: 2.31 ng/mL
standard: 500 ng/mL
Detection range: 7.81-500 ng/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KNG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KNG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KNG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KNG1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01042
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