Rat ALB(Albumin) ELISA Kit

Alternative Names: Serum albumin
Assay Type: Competitive Inhibition
Sensitivity: 0.91 µg/mL
standard: 200 µg/mL
Detection range: 3.13-200 µg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Tumor immunity;Infection immunity;Hematology;Hepatology;Nutrition metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat ALB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02770