Human SCT(Secretin) ELISA Kit

Alternative Names: Secretin
Assay Type: Competitive Inhibition
Sensitivity: 7.01 pg/mL
standard: 1500 pg/mL
Detection range: 23.44-1500 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SCT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCT in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P09683