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ELK Biotechnology
SKU(재고 관리 코드):ELK3032
Mouse POMC(Proopiomelanocortin) ELISA Kit
Mouse POMC(Proopiomelanocortin) ELISA Kit
Alternative Names: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro Opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone
Assay Type: Competitive Inhibition
Sensitivity: 47.8 pg/mL
standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse POMC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse POMC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse POMC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01193
Assay Type: Competitive Inhibition
Sensitivity: 47.8 pg/mL
standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse POMC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse POMC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse POMC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01193
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