1
/
의
1
ELK Biotechnology
SKU(재고 관리 코드):ELK4864
Rat NES1(Nesfatin 1) ELISA Kit
Rat NES1(Nesfatin 1) ELISA Kit
Alternative Names: Nesfatin-1
Assay Type: Competitive Inhibition
Sensitivity: 183.3 pg/mL
standard: 40000 pg/mL
Detection range: 625-40000 pg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Glycolipid metabolism
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NES1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 183.3 pg/mL
standard: 40000 pg/mL
Detection range: 625-40000 pg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Glycolipid metabolism
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NES1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Share
