Rat FGF7(Fibroblast Growth Factor 7) ELISA Kit

Alternative Names: KGF; HBGF7; Keratinocyte Growth Factor; Heparin-binding growth factor 7
Assay Type: Competitive Inhibition
Sensitivity: 5.22 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Developmental science;Pulmonology;Dermatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat FGF7 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGF7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q02195