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ELK Biotechnology
SKU(재고 관리 코드):ELK6090
Mouse NPFF(Neuropeptide FF) ELISA Kit
Mouse NPFF(Neuropeptide FF) ELISA Kit
Alternative Names: FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF
Assay Type: Competitive Inhibition
Sensitivity: 3.69 pg/mL
standard: 500 pg/mL
Detection range: 7.82-500 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NPFF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPFF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9WVA8
Assay Type: Competitive Inhibition
Sensitivity: 3.69 pg/mL
standard: 500 pg/mL
Detection range: 7.82-500 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NPFF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPFF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9WVA8
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