LA(Lactic Acid) ELISA Kit

Alternative Names: LA
Assay Type: Competitive Inhibition
Sensitivity: 2.96 µg/mL
standard: 600 µg/mL
Detection range: 9.38-600 µg/mL
Sample type: serum, plasma and biological fluids
Assay length: 2.5h
Research Area: Enzyme & Kinase;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LA in the samples is then determined by comparing the OD of the samples to the standard curve.