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ELK Biotechnology
SKU(재고 관리 코드):ELK8239
VMA(Vanillylmandelic Acid) ELISA Kit
VMA(Vanillylmandelic Acid) ELISA Kit
Alternative Names: Vanillyl Mandelic Acid
Assay Type: Competitive Inhibition
Sensitivity: 43 ng/mL
standard: 10000 ng/mL
Detection range: 156.25-10000 ng/mL
Sample type: urine and biological agents
Assay length: 2.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VMA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 43 ng/mL
standard: 10000 ng/mL
Detection range: 156.25-10000 ng/mL
Sample type: urine and biological agents
Assay length: 2.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VMA in the samples is then determined by comparing the OD of the samples to the standard curve.
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