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ELK Biotechnology
SKU(재고 관리 코드):ELK8250
PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit
PIP3(Phosphatidylinositol Trisphosphate) ELISA Kit
Alternative Names: PtdIns(3,4,5)P3
Assay Type: Competitive Inhibition
Sensitivity: 2.84 ng/mL
standard: 500 ng/mL
Detection range: 7.82-500 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Signal transduction;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 2.84 ng/mL
standard: 500 ng/mL
Detection range: 7.82-500 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Signal transduction;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
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