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ELK Biotechnology
SKU(재고 관리 코드):ELK9024
Human CNCbl(Cyanocobalamin) ELISA Kit
Human CNCbl(Cyanocobalamin) ELISA Kit
Alternative Names: VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide
Assay Type: Competitive Inhibition
Sensitivity: 48.2 pg/mL
standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;Nutrition metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CNCbl protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 48.2 pg/mL
standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;Nutrition metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CNCbl protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.
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