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ELK Biotechnology
SKU(재고 관리 코드):ELK9448
10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit
10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit
Alternative Names: 10-HETE
Assay Type: Competitive Inhibition
Sensitivity: 3.77 ng/mL
standard: 800 ng/mL
Detection range: 12.5-800 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Enzyme & Kinase;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 10-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 10-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 10-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 3.77 ng/mL
standard: 800 ng/mL
Detection range: 12.5-800 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Enzyme & Kinase;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 10-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 10-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 10-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
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