EnzyChrom™ Superoxide Dismutase Assay Kit

Application

• For quantitative determination of superoxide dismutase (SOD) enzyme activity and evaluation of drug effects.

Key Features

• Sensitive and accurate. Linear detection range of 0.05 – 3 U/mL SOD.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD440nm

Samples

• Blood, cell, tissue, etc

Species

• All

Procedure

• 1 hr

Size

• 100 tests

Detection Limit

• 0.05 U/mL

Shelf Life

• 6 months

More Details

Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O₂ and H₂O₂. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders, and cancer. BioAssay Systems SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O₂^–) is provided by a xanthine oxidase (XO) catalyzed reaction. O₂^– reacts with a WST-1 dye to form a colored product. SOD scavenges the O₂^– thus less O₂^– is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.