Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human TACD1. AA range:116-165
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
34kD
GeneID（Human）:
4072
Human Swiss-Prot No:
P16422
Cellular localization:
Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .
Background:
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008],
Western Blot analysis of various cells using EP-CAM Polyclonal Antibody diluted at 1:2000
Western Blot analysis of 3T3 cells using EP-CAM Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from Jurkat, HUVEC, and COLO205 cells, using TACD1 Antibody. The lane on the right is blocked with the synthesized peptide.