Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human EFNB1/2. AA range:284-333
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
59kD
GeneID（Human）:
1947/1948
Human Swiss-Prot No:
P98172/P52799
Cellular localization:
Cell membrane ; Single-pass type I membrane protein . Membrane raft . May recruit GRIP1 and GRIP2 to membrane raft domains. .; [Ephrin-B1 C-terminal fragment]: Cell membrane ; Single-pass type I membrane protein .; [Ephrin-B1 intracellular domain]: Nucleus . Colocalizes with ZHX2 in the nucleus. .
Background:
The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using Ephrin-B1/2 Polyclonal Antibody diluted at 1:500
Western Blot analysis of 293 cells using Ephrin-B1/2 Polyclonal Antibody diluted at 1:500
Immunohistochemistry analysis of paraffin-embedded human testis tissue, using EFNB1/2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 5', using EFNB1/2 Antibody. The lane on the right is blocked with the synthesized peptide.