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ELK Biotechnology
SKU(재고 관리 코드):EPT030
Recombinant 2019-nCoV S Protein RBD-SD1 (C-6His)
Recombinant 2019-nCoV S Protein RBD-SD1 (C-6His)
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Description: Recombinant SARS-CoV-2 S Protein RBD-SD1 is produced by our Mammalian expression system and the target gene encoding Arg319-Ser591 is expressed with a 6His tag at the C-terminus.
Accession: QHD43416.1
Molecular weight: 31.4kDa
Apparent molecular weight: 35-43kDa, reducing conditions
Other names: 2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein
Storage condition: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity: Greater than 95% as determined by reducing SDS-PAGE/SEC-HPLC.
Biological activity: Immobilized SARS-CoV-2 S Protein RBD-SD1-His (Cat#DRA42) at 5μg/ml (100 μl/well) can bind Human ACE-2-Fc(Cat#C05Y).The ED50 of Recombinant Human ACE-2-Fc(Cat#C05Y) is 76 ng/ml.
Storage: Reconstituted protein solution should be stored at ≤ -20°C.
Delivery condition: The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.
Background: The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Accession: QHD43416.1
Molecular weight: 31.4kDa
Apparent molecular weight: 35-43kDa, reducing conditions
Other names: 2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein
Storage condition: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity: Greater than 95% as determined by reducing SDS-PAGE/SEC-HPLC.
Biological activity: Immobilized SARS-CoV-2 S Protein RBD-SD1-His (Cat#DRA42) at 5μg/ml (100 μl/well) can bind Human ACE-2-Fc(Cat#C05Y).The ED50 of Recombinant Human ACE-2-Fc(Cat#C05Y) is 76 ng/ml.
Storage: Reconstituted protein solution should be stored at ≤ -20°C.
Delivery condition: The product is shipped on dry ice pack.Upon receipt, store it immediately at the temperature listed below.
Background: The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
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