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ELK Biotechnology

SKU(재고 관리 코드):EPT121

Recombinant Mouse HGF (C-6His)

Recombinant Mouse HGF (C-6His)

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Description: Recombinant Mouse Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln33­Leu728 is expressed with a 6His tag at the C-terminus.
Accession: Q08048
Molecular weight: 53.5&26.9 KDa
Apparent molecular weight: 32-38&51-59 KDa, reducing conditions
Other names: Hepatopoietin-A; Scatter factor; SF; deafness; autosomal recessive 39; DFNB39; F-TCF; hepatocyte growth factor
Storage condition: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Delivery condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Background: HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis.  It can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions .
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