ESR2; ESTRB; NR3A2; Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2
Applications:
WB;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-beta around the phosphorylation site of Ser105. AA range:71-120
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
59kD
GeneID（Human）:
2100
Human Swiss-Prot No:
Q92731
Cellular localization:
Nucleus .
Background:
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008],
Western Blot analysis of HepG2 cells using Phospho-ERβ (S105) Polyclonal Antibody
Immunofluorescence analysis of HUVEC cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The lane on the right is blocked with the phospho peptide.