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Exazym® ClickChem Conjugation Kit

Exazym® ClickChem Conjugation Kit

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Reagents intended for conjugation of an oligo-dT primer to a secondary antibody using Click Chemistry.

Product Specification

The reagents required for conjugation of oligo-dT primer to 50 µg of a secondary antibody using Click Chemistry. Exazym® ClickChem Conjugation Kit 50 is designed to be used together with Exazym® Polymerase Reaction Kit 96 or 960 and Exazym® Biotin Detection Kit 96 or 960 and can bring ultra-sensitive detection levels to conventional immunodiagnostic assays.

 

Components

  • 1 vial 75 µg lyophilised Exazym® oligo-dT Primer (DBCO-PEG-30-mer)
  • 1 vial 3 ml DMSO, anhydrous
  • 2 Spin Columns
  • 1 vial 5 mg evaporated ClickChem Label (NHS-PEG4-azide)
  • 1 vial Quenching Buffer
  • 1 tablet PBS-T to be reconstituted in 100ml distilled water

 

Shipping conditions

Shipped on wet ice and ambient temperature.

 

Storage conditions 

  • Exazym® Oligo-dT primer is stored at -30 to -15 °C. Store aliquots of primer dissolved in PBS-T at -20°C.
  • DMSO is stored at 15 to 30 °C.  The vial is for single use only and any remaining DMSO should be discarded.
  • Spin Columns are stored at 15 to 30 °C.
  • ClickChem Label comes evaporated and is stored at -30° to -15°C.
  • Quenching Buffer is stored at -30° to -15°C
  • PBS-T blister tablet is stored at 15 to 30 °C. Prepared PBS-T solution is stored at 2 to 8 °C.

 

Shelf life

18 months from date of manufacture. Please refer to the secondary kit packing label for the manufacturing date.

 

Performance

It is recommended that the conjugation of the detector antibody is performed using molar excess of Click Chem Label versus detector antibody, and then in a second step, molar excess of oligo-dT primer versus azide-labeled detector antibody. The conjugation may be evaluated using SDS-PAGE electrophoresis. A typical result may look like the gel below.

  1. Molecular weight standard
  2. Detector antibody (mAb)
  3. mAb-oligo-dT 5:5
  4. mAb-oligo-dT 20:20
  5. mAb-oligo-dT 40:40

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