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Cavidi

10-0002-01

Exazym® StreptaClick® Primer

Exazym® StreptaClick® Primer

Product Details

Product Specification

The product contains the streptavidin-oligo-dT primer required for polymerization of the DNA:RNA hybrid-ladder using the Exazym® Polymerase Reaction Kit. The reagent supplied is sufficient for at least one 96-well microplate. The Exazym® system can bring ultra-sensitive detection levels to conventional immunodiagnostic assays.

 

Components

  • 1 vial x 2.5 µg Exazym® StreptaClick® Primer, 0.1 mg/mL in TE buffer.

 

Shipping conditions

Shipped on wet ice.

 

Materials and equipment required but not included

  • Exazym® Polymerase Reaction Kit 96 or 960
  • Exazym® Detection Kit 96 or 960 (select the detection moiety appropriate for your assay; see options above)
  • Exazym® Wash Buffer, which is provided as blister tablets with Exazym® Detection Kit 96 and 960.
  • ELISA diluent for dilution of Exazym® StreptaClick® Primer (e.g., PBS-based buffer at neutral pH containing stabilizer and detergent)
  • Adhesive plate covers
  • Plate washer; automated or manual (e.g., multi-pipette)
  • Pipettes and other standard laboratory equipment

Storage conditions

  • Exazym® StreptaClick® Primer is stored at +2 °C to +8 °C.

 

Performance

Exazym® StreptaClick® Primer (cat no. 10-0002-01) integrates BOLD signal amplification into any sandwich immunoassay that uses a biotinylated detector antibody. The data below illustrate typical resulting sensitivity gains on two commercial cytokine ELISAs.

Figure 1

Figure 1. Standard curves and limit-of-detection improvement on two commercial assays. Using the optimized conditions, StreptaClick® Primer was applied to the R&D Systems Human IL-23 DuoSet (cat. DY1290, panel A) and the Mabtech Human IL-4 ELISA Flex (cat. 3410-1H-6, panel B). Both standard curves shifted markedly downward on the concentration axis, with LOD (signal-to-noise = 2) improving 11-fold for IL-23 and 16-fold for IL-4.

 

 

Figure 2

Figure 2. In-assay optimization and resulting signal-to-noise ratio improvements. Three dilutions of the biotinylated detector antibody were each combined with a high and a low concentration of StreptaClick® Primer; signal-to-noise was evaluated at the manufacturer’s lower specification limit (2 pg/mL) and benchmarked against the unmodified standard ELISA. An 8-fold dilution of detector antibody combined with the higher StreptaClick® Primer concentration delivered the best low-end signal-to-noise ratio.

 

 

The degree of sensitivity gain in any assay depends on the extent to which specific signal is amplified as compared to background noise— assays with low intrinsic background see the largest improvements.

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