Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human FGF22. AA range:71-120
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
20kD
GeneID（Human）:
27006
Human Swiss-Prot No:
Q9HCT0
Cellular localization:
Secreted .
Background:
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The mouse homolog of this gene was found to be preferentially expressed in the inner root sheath of the hair follicle, which suggested a role in hair development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014],
Western Blot analysis of various cells using FGF-22 Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of MCF7 cells, using FGF22 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using FGF22 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat and HT-29 cells, using FGF22 Antibody. The lane on the right is blocked with the synthesized peptide.