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SKU(재고 관리 코드):LAA153Ra81

FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)

FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)

UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA153Ra81

Organism Species

Rattus norvegicus (Rat).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

 

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against AFP. It has been selected for its ability to recognize AFP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

BioFactors

Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761

Food and Nutrition Sciences

Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. Scirp: 5684

Diabetes Research and Clinical Practice

Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats ScienceDirect: S0168822711000520

Life Sciences

Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700

Annals of Hepatology

Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. Hepatology: source

Biomedicine & Aging Pathology

Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats ScienceDirect: S2210522013000348

European journal of pharmacology

Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413

Asian Pac J Cancer Prev.

Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529

Journal of Analytical Atomic Spectrometry

Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source

PLOS ONE

Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma Pubmed:24927126

BMC Med Imaging

Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma PubMed: 25981587

Tumour Biol

Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma PubMed: 26643893

Acta Veterinaria Brno

The concentrations of selected blood serum proteins in calves during the first three months of life 85:33

Hepatobiliary Pancreat Dis Int

Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml

Journal of Applied Pharmaceutical Science

Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf

Frontiers in Physiology 

Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats v04n07a07a

International Journal of Clinical and Experimental Medicine

Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model 1345-1349

Tumor Biology

Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway pubmed:28651490

Advances in Clinical and Experimental Medicine

Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles ISSN 2451-2680

Tumor biology

Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats pubmed:28720064

EBioMedicine

Lnc-PCDH9-13: 1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular Carcinoma Pubmed:30045829

Sensors and Actuators B: Chemical

One-pot synthesis of a highly selective carboxyl-functionalized superparamagnetic probes for detection of alpha-fetoprotein 10.1016:j.snb.2018.03.038

Biomedicine & Pharmacotherapy

Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302

Journal of Investigative Surgery

Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation Pubmed:28107094

Asian Pacific Journal of Cancer Prevention

Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats Pubmed:29693337

Antioxid Redox Signal

The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma Pubmed:29687726

Cancer Biology

Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer

Cancer Biology

Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine

Biomedicine & Pharmacotherapy

The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB Pubmed: 30784932

Asian Journal of Pharmacy and Pharmacology

Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma

American Journal of Transplantation

Circulating exosomal miR‐92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence Pubmed: 31162867

Environ Toxicol Pharmacol.

Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats Pubmed: 31421311

Hepatology Research

Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition Pubmed: 31652385

REDOX REPORT

Selective cytotoxic activity and protective effects of sodium ascorbate against hepatocellular carcinoma through its effect on oxidative stress and apoptosis in vivo and … Pubmed: 32172678

Scientific Reports

The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440

Nutr Cancer

Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation Pubmed: 32718197


Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles

Microchemical Journal

Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS


Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats


Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo

DEVELOPMENT

Blocking estrogen-induced AMH expression is crucial for normal follicle formation 33658225

Cytokine

QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model 34564023

Journal of Gastrointestinal Cancer

In Vivo Study of a Newly Synthesized Chromen-4-One Derivative as an Antitumor Agent Against HCC 34698995


Catalog No.

Related products for research use of Rattus norvegicus (Rat) Organism species

Applications (RESEARCH USE ONLY!)

RPA153Ra01

Recombinant Alpha-Fetoprotein (AFP)

Positive Control; Immunogen; SDS-PAGE; WB.

PAA153Ra01

Polyclonal Antibody to Alpha-Fetoprotein (AFP)

WB; IHC; ICC; IP.

LAA153Ra81

FITC-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)

WB; IHC; ICC; IF.

LAA153Ra71

Biotin-Linked Polyclonal Antibody to Alpha-Fetoprotein (AFP)

WB; IHC; ICC.

MAA153Ra21

Monoclonal Antibody to Alpha-Fetoprotein (AFP)

WB; IHC; ICC; IP.

SEA153Ra

ELISA Kit for Alpha-Fetoprotein (AFP)

Enzyme-linked immunosorbent assay for Antigen Detection.

LMA153Ra

Magnetic Luminex Assay Kit for Alpha-Fetoprotein (AFP) ,etc.

Magnetic Luminex Assay for Antigen Detection.

 

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