SKU(재고 관리 코드):LAA565Hu81
FITC-Linked Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG)
FITC-Linked Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA565Hu81
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against HSPG. It has been selected for its ability to recognize HSPG in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Magazine |
Citations |
Journal of Applied Pharmaceutical Science |
Cytotoxic effects of antiglypican-3 against HepG3 cell line Ebscohost: Source |
Journal of Reproductive Immunology |
Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patients Pubmed: 24661950 |
European journal of pharmacology |
Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413 |
European Journal of Pharmacology |
Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma Pubmed:25449037 |
Shock |
Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance Pubmed:25692257 |
Biomarkers in Medicine |
Endothelial glycocalyx layer shedding following lung resection pubmed:27643669 |
Apmis |
Circulating syndecans during critical illness. pubmed:28256016 |
Biomedicine & Pharmacotherapy |
Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302 |
Drug Delivery |
Glioma targeting peptide modified apoferritin nanocage Pubmed:29726297 |
Anaesthesia |
Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass Pubmed: 30687934 |
Animals |
Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period … |
LIFE SCIENCES |
Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients 33781826 |
Genet Mol Biol |
A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate 34042151 |
Poultry Science |
Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡ 33518110 |
J Gerontol A Biol Sci Med Sci |
The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo … 34423832 |
Catalog No. |
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