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SKU(재고 관리 코드):LAA084Hu81

FITC-Linked Polyclonal Antibody to Leptin (LEP)

FITC-Linked Polyclonal Antibody to Leptin (LEP)

UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA084Hu81

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

European Journal of Pharmacology

Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961

Indian Journal of Pharmacology

Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats Ijp: source

Journal of endocrinology

Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit PubMed: 22010203

PLoS One.

Multi-Functional Roles of Chitosan as a Potential Protective Agent against Obesity PubMed: 23342013

Molecular and Cellular Endocrinology

Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194

Journal of Animal and Feed Sciences

Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores Ifzz: Source

British Journal of Nutrition

n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577

Int J Mol Sci.

Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781

Endocr J.

CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658

Animal Physiology

EFFECT OF LEPTIN GENE ON SERUM LEPTIN CONCENTRATION AND BEEF QUALITY IN CZECH FLECKVIEH BULLS Mendelu:Source

Evid Based Complement Alternat Med.

Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645

general endocrinology

Oxytocin reverse ovariectomy-induced Osteoper and body fat gain Pubmed:24506069

Respiratory Physiology & Neurobiology

Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring Pubmed:25500158

Sci Rep

Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430

Evid Based Complement Alternat Med

Tinospora crispa PubMed: 25821506

Ankara

Effects of breeder age and early energy restriction on fattening performance, some meat quality traits and plasma leptin concentration of broilers Dergiler: 11

MEDICINA VETERIN

Comparative measurements of the morphometric pattern and energy profile of Crioula mares in the final third of gestation, with different body condition scores Scielo: S0102-09352015000300707

Pancreatic Disorders & Therapy

Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrients and Growth, but Also Body Composition and Endocrinological Parameters – Study on Piglets Used As a Model for Children Journals: 2165-7092-%20S5-00

Veterinary Word

Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212

Animal

Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes. Pubmed:27074715

ANNALS OF ANIMAL SCIENCE

Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87

Annals of Animal Science

Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87

Obes Surg.

Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175

Acta Histochemica

Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234

J Biomed Mater Res A

Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice Pubmed:26850371

Clin Epigenetics.

The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model Pubmed:27110299

International Journal of Molecular Sciences

Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency pubmed:27690005

Journal of Animal Science

Changes in small intestinal morphometry and electrogenic nutrient transport in young pigs with pancreatic exocrine insufficiency publications:jas

International Journal of Endocrinology

The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162

journal of animal science

The effect of GnRH vaccination on performance, carcass, and meat quality and hormonal regulation in boars, barrows, and gilts. pubmed:27482668

journal of animal science

Diet-induced modifications to milk composition have long-term effects on offspring growth in rabbits. pubmed:28380600

Evidence-Based Complementary and Alternative Medicine

Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535

Asian-Australasian Journal of Animal Sciences

Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817

Stem Cell Research & Therapy

Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizingradiation. pubmed:27530249

Obesity Surgery

Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in ObeseGöttingen Minipig. Effects on Glucose Metabolism. pubmed:27185175

Meat Sci

Estimation of the intramuscular fat content of m. longissimus thoracis in crossbred beef cattle based on live animal measurements. pubmed:27940421

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY

Correlation of Leptin, Insulin and Glucose During Late Gestation and Early Lactation in Murrah Buffaloes 7086

Journal of Animal and Plant Sciences

Effects Of Maca (Lepıdıum Meyenıı Walp) Powder On Serum Indıces And Metabolıc Responses In Racehorses 57cf073008ae83b37462323a.pdf

Biological Trace Element Research

Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007

Revista Brasileira de Ciência Avícola

Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285

Applied Animal Behaviour Science

Evaluating measures of exploratory behaviour in sows around farrowing and during lactation− A pilot study 10.1016/j.jacl.2017.03.007

Proceedings of the National Academy of Sciences of the United States of America (PNAS)

cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis Pubmed:29784793

Oxidative Medicine and Cellular Longevity

Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … Pubmed:29849871

Frontiers in Endocrinology

Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … Pubmed:29755407

Journal of Agricultural and Food Chemistry

Metabolomics for the effect of biotin and nicotinamide on transition dairy cows Pubmed:29758980

Frontiers in Physiology

Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555

Bioscience Reports

Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Pubmed:29784871

Neurochemical Research

The leptin, dopamine and serotonin receptors in hypothalamic POMC-neurons of normal and obese rodents Pubmed:29397535

PLoS One

Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588

Biochemical and Biophysical Research Communications

Chronic cold exposure results in subcutaneous adipose tissue browning and altered global metabolism in Qinghai-Tibetan plateau pika (Ochotona curzoniae) Pubmed:29626477

Alcohol and Alcoholism

Leptin Expression and Gene Methylation Patterns in Alcohol-Dependent Patients with Ethyltoxic Cirrhosis—Normalization After Liver Transplantation and Implications … Pubmed:29912265

Iranian Red Crescent Medical Journal 

The Effect of Aerobic Training on Tumor Growth, Adiponectin, Leptin and Ghrelin in Mice with Breast Cancer ircmj-20-03-13305.pdf

Pakistan Journal of Zoology

Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. :

Oncotarget

Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120

УСПЕХИ ГЕРОНТОЛОГИИ

ВЛИЯНИЕ МЕТФОРМИНА НА МЕТАБОЛИЧЕСКИЕ ПОКАЗАТЕЛИ И ГИПОТАЛАМИЧЕСКИЕ СИГНАЛЬНЫЕ СИСТЕМЫ У КРЫС С ОЖИРЕНИЕМ … AG_2018-31-01.pdf

Food Science and Technology Research

Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 Cells Doi: 10.3136/fstr.24.903

Nutrients

Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet Pubmed: 30400147

Psychopharmacology

Betahistine effects on weight-related measures in patients treated with antipsychotic medications: a double-blind placebo-controlled study Pubmed: 30382354

Progress in Nutrition

Effect of fermented food consumption on biochemical parameters and adipokines levels Doi: 0.23751/pn.v20i4.7374

THE INDIAN JOURNAL OF VETERINARY SCIENCES & BIOTECHNOLOGY

THE INDIAN JOURNAL OF VETERINARY SCIENCES & BIOTECHNOLOGY

Biomedicine & Pharmacotherapy

Raspberry ketone and Garcinia Cambogia rebalanced disrupted insulin resistance and leptin signaling in rats fed high fat fructose diet Pubmed: 30530230

Nutrition in the Prevention and Treatment of Abdominal Obesity

Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig

Scientific Reports

Obesity Aggravates Acute Pancreatitis via Damaging Intestinal Mucosal Barrier and Changing Microbiota Composition in Rats Pubmed: 30635594

Atherosclerotic diseases

Cardiotrophin-1 and leptin as cardiovascular risk markers in male patients with obstructive sleep apnea syndrome Pubmed: 30775602

PLoS One

Impact of exposure to diesel exhaust during pregnancy on mammary gland development and milk composition in the rabbit Pubmed: 30763367

Scientific Journal of Kurdistan University of Medical Sciences

Effect of High Intensity Interval Training (HIIT) on visceral and subcutaneous levels of leptin and plasma insulin and glucose in male Wistar rats

PLOS ONE

The evidence of metabolic-improving effect of metformin in Ay/a mice with genetically-induced melanocortin obesity and the contribution of hypothalamic … Pubmed: 30870482

International Journal of Pharmaceutical Research & Allied Sciences

Osteoprotegerin Levels among Saudi Cardiovascular Patients.

Journal of Ethnopharmacology

Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet

Gene

Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertension Pubmed: 30940525

中国药理学通报

冷暴露对高脂饮食小鼠脂肪棕色化的影响

Arch Physiol Biochem

Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training Pubmed: 31290696

Food & Function

A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese …

Nutrients

High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …

Developmental Dynamics

Puberty is a critical window for the impact of diet on mammary gland development in the rabbit Pubmed: 31348557

Adv Gerontol

The Effect of Different Types of Bariatric Surgery on Metabolic and Hormonal Parameters in Rats with a Decompensated Form of Type II Diabetes Mellitus Pubmed: 31228372

Proceedings of the Royal Society B: Biological Sciences

Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickens Pubmed: 31506054

Food & Function

Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese Mice Pubmed: 31670366

journal of ethnopharmacology

Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model Pubmed: 31756448

Annals of Nutrition and Metabolism

Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with Obesity Pubmed: 31775140

Journal of Ethnopharmacology

Upregulation of MC4R and PPAR-α expression mediates the anti-obesity activity of Moringa oleifera Lam. in high-fat diet-induced obesity in rats Pubmed: 31911179

Journal of Natural Products

Prenylated Phenolic Compounds from the Aerial Parts of Glycyrrhiza uralensis as PTP1B and α-Glucosidase Inhibitors Pubmed: 32196343

gynecological endocrinology

Salivary Leptin and Chemerin; a novel way of gestational diabetes screening Pubmed: 32274942

Frontiers in Endocrinology

RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis Pubmed: 32328034

Ann Nutr Metab

Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity Pubmed: 33027789

ANDROLOGIA

The effect of metformin treatment on the basal and gonadotropin‐stimulated steroidogenesis in male rats with type 2 diabetes mellitus Pubmed: 32951228

GENERAL AND COMPARATIVE ENDOCRINOLOGY

Adipokines expression profiles in both plasma and peri renal adipose tissue in Large White and Meishan sows: a possible involvement in the fattening and … Pubmed: 32827511

Nutrition & Diabetes

C-Peptide and leptin system in dichorionic, small and appropriate for gestational age twins—possible link to metabolic programming? Pubmed: 32778645

BIOLOGICAL TRACE ELEMENT RESEARCH

Ameliorative Effect of Ananas comosus on Cobalt Chloride-Induced Hypoxia in Caco2 cells via HIF-1α, GLUT 1, VEGF, ANG and FGF Pubmed: 32654099

plos one

Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysis Pubmed: 30089154

Journal of reproduction and fertility

Blastomere removal affects homeostatic control leading to obesity in male mice Pubmed: 33112292

Journal of Evolutionary Biochemistry and Physiology

The Effect of Diet-Induced and Melanocortin Obesity on Expression of Tryptophan Hydroxylase 2 in the Dorsal Raphe Nucleus and Ventral Tegmental Area in Mice

Indian Journal of Animal Sciences

Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …


СОСТОЯНИЕ МЕЛАТОНИН-АССОЦИИРОВАННОЙ ГОРМОНАЛЬНОЙ СИСТЕМЫ У ДЕТЕЙ С ИЗБЫТОЧНОЙ МАССОЙ ТЕЛА

biorxiv

Altered hematopoietic system and self-tolerance in Bardet-Biedl Syndrome

The Egyptian Journal of Neurology, Psychiatry and Neurosurgery volume

Serum leptin and body mass index in a sample of Egyptian multiple sclerosis patients

Biosci Rep

A 12-week aerobic exercise improves metabolic function with lower adipose tissue and ectopic fat in high fat diet fed rats 33432988

Cell Metab

TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease 34107313

International Journal of Endocrinology

Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­ 34035808

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY

Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats

The pharma innovation Journal

Comparative study on genital status, energy balance and mineral content of swamp buffalo (Luit) heifers and cows under organized system of rearing in Assam?¡­

Indian Journal of Forensic Medicine & Toxicology

Altered Serum Marker of Adipokines Profile in Breast Cancer Women

PPAR Research

Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment … 34306045

Br J Nutr

The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study 34348809

Ann Med Surg (Lond)

Maternal and fetal serum leptin levels and their association with maternal and fetal variables and labor: A cross-sectional study 34815864

Biofactors

Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat 34767670

Nat Commun

GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity 34853313

Int J Mol Sci

The Effects of Separate and Combined Treatment of Male Rats with Type 2 Diabetes with Metformin and Orthosteric and Allosteric Agonists of Luteinizing Hormone … Pubmed:35008624

Bull Exp Biol Med

Comparative Study of the Restoring Effect of Metformin, Gonadotropin, and Allosteric Agonist of Luteinizing Hormone Receptor on Spermatogenesis in Male Rats with … Pubmed:35175480

Naunyn Schmiedebergs Arch Pharmacol

Intragastric safflower yellow and its main component HSYA improve leptin sensitivity before body weight change in diet-induced obese mice Pubmed:35201390

npj Biofilms and Microbiomes

The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal Pubmed:35379849

Ecotoxicol Environ Saf

Monochromatic blue light not green light exposure is associated with continuous light-induced hepatic steatosis in high fat diet fed-mice via oxidative stress Pubmed:35588616

Biomed Res Int

Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model Pubmed:35509713

Endocr J

A novel anti-obesity mechanism for liraglutide by improving adipose tissue leptin resistance in high-fat diet-fed obese mice Pubmed:35705299


Impact of pre-breeding feeding practices on rabbit mammary gland development at mid-pregnancy.


High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients


Dairy Cattle Physiology Division ICAR-National Dairy Research Institute, Karnal-132001, Haryana, India Corresponding Authors Email ID: menalsh08vets …

Scientific Reports

SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.


Catalog No.

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Applications (RESEARCH USE ONLY!)

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Positive Control; Immunogen; SDS-PAGE; WB.

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WB; IHC; ICC; IP.

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WB; IHC; ICC.

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WB; IHC; ICC; IF.

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WB; IHC; ICC; IP.

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WB

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WB; IHC; ICC; IP.

MAA084Hu24

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WB; IHC; ICC; IP.

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WB; IHC; ICC; IP.

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High Sensitive ELISA Kit for Leptin (LEP)

Enzyme-linked immunosorbent assay for Antigen Detection.

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Enzyme-linked immunosorbent assay for Antigen Detection.

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Mini Samples ELISA Kit for Leptin (LEP)

Enzyme-linked immunosorbent assay for Antigen Detection.

SCA084Hu

CLIA Kit for Leptin (LEP)

Chemiluminescent immunoassay for Antigen Detection.

LMA084Hu

Magnetic Luminex Assay Kit for Leptin (LEP) ,etc.

Magnetic Luminex Assay for Antigen Detection.

KSA084Hu01

ELISA Kit DIY Materials for Leptin (LEP)

Main materials for "Do It (ELISA Kit) Yourself".

 

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