Cloud-clone
SKU(재고 관리 코드):LAA528Hu81
FITC-Linked Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
FITC-Linked Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA528Hu81
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PDGFA. It has been selected for its ability to recognize PDGFA in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Canadian Journal of Physiology and Pharmacology |
The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats pubmed:28177680 |
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International Immunopharmacology |
Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway pubmed:28521243 |
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Open Access Macedonian Journal of Medical Sciences |
Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells |
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Prostaglandins & other Lipid Mediators |
Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway Pubmed: 31698141 |
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Ann Agric Environ Med |
PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis Pubmed: 32208584 |
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Tissue Eng Regen Med |
Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method 34339025 |
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Biomed Research International |
Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form Pubmed:35586817 |
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