Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human FUK. AA range:11-60
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
110kD
GeneID（Human）:
197258
Human Swiss-Prot No:
Q8N0W3
Cellular localization:
cytoplasm,cytosol,
Background:
fucokinase(FUK) Homo sapiens The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date. [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using Fucokinase Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells, using FUK Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, treated with insulin 0.01U/ml 45', using FUK Antibody. The lane on the right is blocked with the synthesized peptide.