Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human GAD1/2. AA range:545-594
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
65kD
GeneID（Human）:
2571/2572
Human Swiss-Prot No:
Q99259/Q05329
Cellular localization:
intracellular,plasma membrane,vesicle membrane,presynaptic active zone,clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane,
Background:
glutamate decarboxylase 1(GAD1) Homo sapiens This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using GAD-65/67 Polyclonal Antibody
Western Blot analysis of mouse cells using GAD-65/67 Polyclonal Antibody
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using GAD1/2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from mouse brain, using GAD1/2 Antibody. The lane on the right is blocked with the synthesized peptide.