Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human GATA4. AA range:228-277
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
48kD
GeneID（Human）:
2626
Human Swiss-Prot No:
P43694
Cellular localization:
Nucleus .
Background:
This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015],
Western Blot analysis of COLO cells using GATA-4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunofluorescence analysis of HUVEC cells, using GATA4 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO205 cells, treated with Serum 20% 15', using GATA4 Antibody. The lane on the right is blocked with the synthesized peptide.