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ELK Biotechnology

ELK9583

General TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit

General TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit

Sensitivity

General TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit

Overview

Product name: TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit
Reactivity: General
Alternative Names: Cartilage-inducing factor;CED;Differentiation inhibiting factor;DPD1;LAP;Latency-associated peptide;Prepro transforming growth factor beta 1;TGF beta 1;TGF beta;TGF beta 1 protein;TGF-beta 1 protein;TGF-beta-1;TGF-beta-5;TGF-beta1;TGFB;Tgfb-1;tgfb1;TGFB1;TGFbeta;TGFbeta1;Transforming Growth Factor b1;Transforming Growth Factor beta 1;Transforming growth factor beta 1a;transforming growth factor beta-1;transforming growth factor;beta 1;Transforming Growth Factor-ß1
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGF-b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGF-b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGF-b1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.266 2.170
5.00 1.694 1.598
2.50 1.184 1.088
1.25 0.892 0.796
0.63 0.582 0.486
0.32 0.389 0.293
0.16 0.256 0.160
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TGF-b1 and the recovery rates were calculated by comparing the measured value to the expected amount of TGF-b1 in samples.
Matrix Recovery range Average
serum(n=5) 79-97% 88%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 96-107% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGF-b1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-96% 93-102% 86-94% 90-98%
EDTA plasma(n=5) 92-106% 85-97% 91-103% 85-97%
Heparin plasma(n=5) 90-99% 82-101% 88-102% 93-104%
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