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ELK Biotechnology
SKU(재고 관리 코드):ES5510
GFAP Rabbit Polyclonal Antibody
GFAP Rabbit Polyclonal Antibody
GFAP Rabbit Polyclonal Antibody
Overview
| Product name: | GFAP rabbit pAb |
| Alternative Names: | GFAP; Glial fibrillary acidic protein; GFAP |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human GFAP. AA range:11-60 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 50kD |
| GeneID(Human): | 2670 |
| Human Swiss-Prot No: | P14136 |
| Cellular localization: | Cytoplasm . Associated with intermediate filaments. . |
| Background: | This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008], |
| Species Reactivity: | Human;Rat;Mouse; |
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Immunofluorescence analysis of Hela cell. 1,GFAP Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. -
Western Blot analysis of RAT-MUSCLE cells using GFAP Polyclonal Antibody diluted at 1:2000 -
Western Blot analysis of RAW using GFAP Polyclonal Antibody diluted at 1:2000 -
Immunofluorescence analysis of COS7 cells, using GFAP Antibody. The picture on the right is blocked with the synthesized peptide.
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