GPR27; SREB1; Probable G-protein coupled receptor 27; Super conserved receptor expressed in brain 1
Applications:
WB;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human GPR27. AA range:181-230
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
39kD
GeneID（Human）:
2850
Human Swiss-Prot No:
Q9NS67
Cellular localization:
Cell membrane ; Multi-pass membrane protein .
Background:
GPR27 is a member of the G protein-coupled receptors (GPCRs), a large family of receptors that have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters, hormones, or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins.[supplied by OMIM, May 2010],
Immunofluorescence analysis of A549 cells, using GPR27 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HUVEC cells, using GPR27 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HeLa cells using GPR27 antibody.