GRK1; RHOK; Rhodopsin kinase; RK; G protein-coupled receptor kinase 1
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human GRK1 around the phosphorylation site of Ser21. AA range:6-55
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
63kD
GeneID（Human）:
6011
Human Swiss-Prot No:
Q15835
Cellular localization:
Membrane ; Lipid-anchor . Cell projection, cilium, photoreceptor outer segment . Subcellular location is not affected by light or dark conditions. .
Background:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008],
Immunohistochemistry analysis of paraffin-embedded human brain, using GRK1 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GRK1 (Phospho-Ser21) Antibody. The lane on the right is blocked with the phospho peptide.