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ELK Biotechnology

ELK9732

Hamster CTSK(Cathepsin K) ELISA Kit

Hamster CTSK(Cathepsin K) ELISA Kit

Sensitivity

Hamster CTSK(Cathepsin K) ELISA Kit

Overview

Product name: Hamster CTSK(Cathepsin K) ELISA Kit
Reactivity: Hamster
Alternative Names: CTS-K; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD; Pycnodysostosis; Cathepsin O; Cathepsin X
Assay Type: Sandwich
Sensitivity: 5.5 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster CTSK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster CTSK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster CTSK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster CTSK in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
800.00 2.201 2.113
400.00 1.579 1.491
200.00 1.186 1.098
100.00 0.839 0.751
50.00 0.518 0.430
25.00 0.336 0.248
12.50 0.175 0.087
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CTSK and the recovery rates were calculated by comparing the measured value to the expected amount of CTSK in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 80-94% 87%
Heparin plasma(n=5) 90-107% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTSK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 87-101% 89-102% 89-101%
EDTA plasma(n=5) 79-92% 82-93% 83-97% 82-103%
Heparin plasma(n=5) 81-91% 93-106% 89-97% 85-94%
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