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ELK Biotechnology
SKU(재고 관리 코드):ES5743
HMG-14 (phospho Ser21) Rabbit Polyclonal Antibody
HMG-14 (phospho Ser21) Rabbit Polyclonal Antibody
HMG-14 (phospho Ser21) Rabbit Polyclonal Antibody
Overview
| Product name: | HMG-14 (phospho Ser21) rabbit pAb |
| Alternative Names: | HMGN1; HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1 |
| Applications: | IHC;IF;ELISA |
| Recommended Dilutions: | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HMG14 around the phosphorylation site of Ser21. AA range:10-59 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| GeneID(Human): | 3150 |
| Human Swiss-Prot No: | P05114 |
| Cellular localization: | Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus. |
| Background: | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HMG14 (Phospho-Ser21) Antibody -
Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.
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