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ELK Biotechnology

ELK9675

Horse sCD14(soluble cluster of differentiation 14) ELISA Kit

Horse sCD14(soluble cluster of differentiation 14) ELISA Kit

Sensitivity

Horse sCD14(soluble cluster of differentiation 14) ELISA Kit

Overview

Product name: Horse sCD14(soluble cluster of differentiation 14) ELISA Kit
Reactivity: Horse
Alternative Names: CD14 Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD antigen CD14; soluble cluster of differentiation 14
Assay Type: Sandwich
Sensitivity: 0.229 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse sCD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse sCD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse sCD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse sCD14 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.172 2.090
10.00 1.532 1.450
5.00 1.120 1.038
2.50 0.852 0.770
1.25 0.573 0.491
0.63 0.372 0.290
0.32 0.234 0.152
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sCD14 and the recovery rates were calculated by comparing the measured value to the expected amount of sCD14 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 82-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sCD14 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-98% 80-93% 89-97% 89-97%
EDTA plasma(n=5) 93-102% 81-96% 88-97% 86-92%
Heparin plasma(n=5) 95-103% 89-97% 79-94% 87-96%
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