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ELK Biotechnology
SKU(재고 관리 코드):ES2566
HRI Rabbit Polyclonal Antibody
HRI Rabbit Polyclonal Antibody
HRI Rabbit Polyclonal Antibody
Overview
| Product name: | HRI rabbit pAb |
| Alternative Names: | EIF2AK1; HRI; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human EIF2AK1. AA range:571-620 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 71kD |
| GeneID(Human): | 27102 |
| Human Swiss-Prot No: | Q9BQI3 |
| Cellular localization: | cytoplasm, |
| Background: | The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008], |
| Species Reactivity: | Human;Mouse;Monkey |
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Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. -
Western Blot analysis of various cells using HRI Polyclonal Antibody -
Western blot analysis of lysates from A549 and COS7 cells, using EIF2AK1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from COLO205 cells using EIF2AK1 antibody.
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