HSPH1; HSP105; HSP110; KIAA0201; Heat shock protein 105 kDa; Antigen NY-CO-25; Heat shock 110 kDa protein
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human HSP105. AA range:791-840
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
100kD
GeneID（Human）:
10808
Human Swiss-Prot No:
Q92598
Cellular localization:
Cytoplasm .
Background:
function:Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities.,PTM:Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.,similarity:Belongs to the heat shock protein 70 family.,subunit:Interacts with HSPA8/HSC70.,tissue specificity:Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.,
Western Blot analysis of various cells using HSP105 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells, using HSP105 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using HSP105 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using HSP105 Antibody. The lane on the right is blocked with the synthesized peptide.