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FineTest

EH2534

Human 14-3-3 protein eta(YWHAH) ELISA Kit

Human 14-3-3 protein eta(YWHAH) ELISA Kit

size
Sensitivity
Range

Product Name

Human 14-3-3 protein eta(YWHAH) ELISA Kit

 

Alias

YWHA1 ELISA Kit, Protein AS1 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

14-3-3 protein eta ELISA Kit allows for the in vitro quantitative determination of 14-3-3 protein eta concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Species

Human

UniProt ID

Q04917

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 14-3-3 protein eta and the recovery rates were calculated by comparing the measured value to the expected amount of 14-3-3 protein eta in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-101

91

EDTA plasma(n=5)

90-105

99

heparin plasma(n=5)

89-98

94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein eta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

87-103%

90-97%

86-105%

EDTA plasma(n=5)

83-95%

83-101%

85-100%

heparin plasma(n=5)

83-98%

83-98%

83-96%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

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