FineTest
SKU(재고 관리 코드):EH3799
Human 15-HETE (15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Human 15-HETE (15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Product Name
Human 15-HETE (15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Alias
15-HETE ELISA Kit, 15-Hydroxyeicosatetraenoic Acid ELISA Kit
Detection Method
Competitive ELISA, Coated with Antibody
Application
15-HETE ELISA Kit allows for the in vitro quantitative determination of 15-HETE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 15-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 15-HETE in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-102 |
96 |
|
EDTA plasma(n=5) |
85-105 |
95 |
|
heparin plasma(n=5) |
90-101 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
89-103% |
86-99% |
|
EDTA plasma(n=5) |
82-97% |
86-101% |
87-100% |
|
heparin plasma(n=5) |
81-100% |
80-93% |
82-88% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
