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ELK Biotechnology

ELK8060

Human Anti-CRP(Anti-C Reactive Protein Antibody) ELISA Kit

Human Anti-CRP(Anti-C Reactive Protein Antibody) ELISA Kit

Size
Sensitivity
Detection range

Human Anti-CRP(Anti-C Reactive Protein Antibody) ELISA Kit

Overview

Product name: Human Anti-CRP(Anti-C Reactive Protein Antibody) ELISA Kit
Reactivity: Human
Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Assay Type: Competitive Inhibition
Sensitivity: 1.14 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2.5h
Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-CRP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.212 —
100.00 0.386 —
50.00 0.583 —
25.00 0.833 —
12.50 1.156 —
6.25 1.511 —
3.13 1.697 —
0.00 2.226 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-CRP and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-CRP in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-CRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-104% 82-96% 95-102%
EDTA plasma(n=5) 92-104% 82-96% 95-102% 87-98%
Heparin plasma(n=5) 86-97% 90-98% 80-93% 92-105%
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