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ELK Biotechnology

ELK7847

Human Anti-GH(Anti-Growth Hormone Antibody) ELISA Kit

Human Anti-GH(Anti-Growth Hormone Antibody) ELISA Kit

Size
Sensitivity
Detection range

Human Anti-GH(Anti-Growth Hormone Antibody) ELISA Kit

Overview

Product name: Human Anti-GH(Anti-Growth Hormone Antibody) ELISA Kit
Reactivity: Human
Alternative Names: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal
Assay Type: Competitive Inhibition
Sensitivity: 1.12 ng/mL
Standard: 200 ng/mL
Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h, 30min
Research Area: Endocrinology;Neuro science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-GH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.227 —
100.00 0.386 —
50.00 0.575 —
25.00 0.878 —
12.50 1.169 —
6.25 1.537 —
3.13 1.796 —
0.00 2.256 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-GH and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-GH in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-GH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 95-103% 95-103% 87-98%
EDTA plasma(n=5) 92-105% 83-92% 89-98% 85-94%
Heparin plasma(n=5) 79-96% 85-99% 79-93% 89-97%
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