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ELK Biotechnology

ELK8057

Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit

Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit

Size
Sensitivity
Detection range

Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit

Overview

Product name: Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit
Reactivity: Human
Alternative Names: MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
Assay Type: Sandwich
Sensitivity: 2.45 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 2.126 2.037
200.00 1.674 1.585
100.00 1.174 1.085
50.00 0.912 0.823
25.00 0.548 0.459
12.50 0.305 0.216
6.25 0.156 0.067
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-MBL and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-MBL in samples.
Matrix Recovery range Average
serum(n=5) 85-99% 92%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 96-107% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-MBL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 95-103% 95-103% 87-98%
EDTA plasma(n=5) 83-98% 92-103% 86-97% 93-101%
Heparin plasma(n=5) 87-96% 86-98% 91-103% 87-90%
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