Human ATF5(Activating Transcription Factor 5) ELISA Kit

The Human ATF5 (Activating Transcription Factor 5) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human ATF5 in various biological samples. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human ATF5.

The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody against human ATF5 is added, which binds to the captured protein. Streptavidin-HRP conjugate is then added, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured using a microplate reader. The concentration of human ATF5 in the sample is determined by comparing the absorbance of the sample to a standard curve. This kit is highly specific, with no cross-reactivity with other human proteins. It is suitable for use in research and clinical laboratories for the detection and quantification of human ATF5 in various biological samples.